This research project will study radiation-tolerant microorganisms for the purpose of ultimately developing stable biosensors -- chemical sensors that use the accuracy of biological molecules. Researchers will screen specific microbial DNA for useful enzymes that can then be used to develop biologically based sensors for detecting hazardous metals and/or radionuclide contaminants. This research helps DOE in several ways. New sensor technologies should enhance the DOE’s ability to judge the effectiveness of environmental remediation efforts. The research should also contribute to improved understanding of how radiotolerant microbes interact with metals in highly radioactive environments. This could lead to better controlled, more stable long-term storage of high-level nuclear wastes and help prevent unwanted environmental releases.